FIG. 1.

Schematic diagram of PTEN localization mutants. To target PTEN into different subcellular compartments, specific localization signals were inserted into PTEN′s sequence via PCR or molecular cloning as described in Materials and Methods. These were: N-terminal Myr (8-amino-acid [aa] myristoylation motif, MKGSLTTH) targeting the plasma membrane, N-terminal NLS (7 aa, M+RRKKRK) targeting the nucleus, and ER retention motifs (21 N-terminal aa containing an ER signal peptide and 28 C-terminal aa containing an ER retention signal) targeting the ER. In addition, TKV/TVD mutations were introduced to abolish PTEN′s PDZ binding ability.