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Substantiation of PTEN′s localization with subcellular fractionation. Immunoblotting with a MAb to PTEN was performed on cellular fractions extracted from cell lines expressing WT PTEN and U251MG PTEN mutant clones, as indicated (C, cytoplasm; N, nucleus), to corroborate the immunostaining results. Immunoblotting with MAb against poly(ADP-ribose) polymerase 1 (PARP-1), a nucleus-specific protein, showed minimum cross-contamination between the cytoplasmic and nuclear fractions.
