FIG. 6.

Nuclear PTEN does not inhibit cell invasion. (A) A Matrigel invasion assay was performed using a transwell membrane coated with 0.7 mg/ml of Matrigel matrix. Cells were seeded at a concentration of 2 × 10⁵ cells/well overnight. Cells that invaded the bottom side of the membrane were fixed and stained using a hema-3 kit. (B) The number of invaded cells in vector clones was set at 100%. The results shown are the averages of the results from five individual experiments (means ± SD). Only PTEN (WT), ΔPDZB, Myr, and Myr (ΔPDZB) clones showed statistically significant inhibition of cell invasion compared to the vector control (*, P < 10⁻⁶).