FIG. 7.
Nuclear PTEN inhibits p70S6K activation without down-regulating Akt. Cell lysates equivalent to 5 × 105 cells from stable clones grown in the presence of 1% serum for 3 days were loaded into each well on an sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel and transferred onto PVDF membranes. The filters were blotted with antibodies against PTEN, Akt, phospho-Akt (S473), phospho-Akt (T308), p70S6K, phospho-p70S6K (T389), S6, phospho-S6 (S235/236), GSK3β, phospho-GSK3β (S9), and phospho-Erk1/2, respectively, followed by horseradish peroxidase-conjugated secondary antibodies and visualized by the ECL reaction.