FIG. 9.

AMPK is activated by nuclear PTEN. Western blotting was performed on lysates of cells expressing the indicated mutants as described in Materials and Methods using antibodies against Akt, phospho-Akt (S473), p70S6K, phospho-p70S6K (T389), mTOR, phospho-mTOR (S2448), TSC2, phospho-TSC2 (T1462), TSC1, AMPK, phospho-AMPK (T172), S6, phospho-S6 (S235/236), 4E-BP1, phospho-4E-BP1 (T36/46), GSK3β, and phospho-GSK3β (S9), followed by horseradish peroxidase-conjugated secondary antibodies, and visualized by the ECL reaction.