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. 2005 Jul;25(14):6211–6224. doi: 10.1128/MCB.25.14.6211-6224.2005

TABLE 2.

A constitutively active Akt mutant, Akt-DD, only partially rescues nuclear PTEN-mediated growth suppression

U251MG cell type % Soft agar colony formationa % G1 arrestb
Vector 100.0 ± 3.0c 48.7 ± 11.4
NLS-PTEN 2.3 ± 0.5 70.9 ± 6.2
Akt-DD 132.6 ± 7.7 31.1 ± 2.6
NLS-PTEN + Akt-DD (clone 1) 21.1 ± 2.3 60.9 ± 5.1
NLS-PTEN + Akt-DD (clone 2) 13.3 ± 2.6 65.6 ± 5.7
a

Cells (5 × 104/well) were seeded and colonies were measured as described in the legend to Fig. 4A.

b

G1 arrest was measured by flow cytometry as described in the legend to Fig. 5.

c

The percentage of soft agar colonies in vector-transfected U251MG clones was set at 100%.