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. 2005 Jul;25(14):6235–6246. doi: 10.1128/MCB.25.14.6235-6246.2005

FIG. 3.

FIG. 3.

FIG. 3.

FIG. 3.

Effects of TLS-ERG and its mutants on GPIX promoter in L-G and NIH 3T3 cells. (A) Expression of the mouse GPIX gene was examined by RT-PCR in L-G (lane 1) and NIH 3T3 (lane 2) cells. (B) pGL3 or pGL3-GPIX firefly luciferase reporter construct (1.5 μg) was introduced into L-G cells with increasing amounts of pCR3-HA-TLS-ERG as indicated. The total amount of the DNA was kept constant by the addition of pCR3 empty vector. (C) pGL3-GPIX (1.5 μg) was cotransfected into L-G cells with 250 ng of pCR3-HA-TLS-ERG construct expressing TLS-ERG or its mutants. (D) pGL3-GPIX (1.5 μg) was cotransfected into NIH 3T3 cells with 250 ng of pCR3-HA-TLS-ERG construct expressing TLS-ERG or its mutants. (E) pGL3-ESET firefly luciferase reporter construct (1.5 μg) was introduced into L-G and NIH 3T3 cells with increasing amounts of pCR3-HA-TLS-ERG as indicated. All transfections were carried out independently at least three times, and the promoter activity was normalized to that of the Renilla luciferase control.