Venn diagrams of genes affected by TLS-ERG and its mutants in L-G and NIH 3T3 cells. (A) RNAs from L-G and NIH 3T3 cells stably expressing HA-TLS-ERG were used to analyze gene expression patterns on the Affymetrix GeneChips (mouse genome 430, version 2.0, array). Probe sets that differed from the HA-TLS-ERGΔETS baseline control by at least twofold were selected. The sizes of the Venn circles are proportional to the total number of modulated probe sets. Venn numbers correspond to overlapped probe sets between L-G and NIH 3T3 cells. RNAs from L-G (B) and NIH 3T3 (C) cells stably expressing HA-TLS-ERG, HA-TLS-ERGΔ1-173, or HA-TLS-ERGΔ174-265 were analyzed by the mouse genome 430, version 2.0, array using HA-TLS-ERGΔETS as the baseline control. The total number of probe sets that were affected twofold or higher by each construct is included in parentheses. The number of probe sets shared by the mutants is also indicated.