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. 2005 Jul;25(14):6112–6122. doi: 10.1128/MCB.25.14.6112-6122.2005

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FIG. 3. — Binding of talin-VBS1, talin-VBS3, or αVBS peptides to vinculin's head domain (VH, human residues 1 to 780) is sufficient to displace Vt from preexisting VH-Vt complexes. Native polyacrylamide gel electrophoresis analysis of free VH (lane 1) and of VH bound to talin-VBS1 (lane 2), talin-VBS3 (lane3), αVBS (lane 4), or Vt (human vinculin residues 879 to 1066; lane 5) is shown. Free VH is indicated with an asterisk. Talin-VBS1, talin-VBS3, and αVBS were then added to preexisting VH-Vt complexes (circle) at a fourfold molar excess and displaced Vt to form the respective VH-talin-VBS1 (lane 6), VH-talin-VBS3 (lane 7), or VH-αVBS (lane 8). Free Vt is not visible in native gels because of its basic pI.