FIG. 6.

uPA expression in PC3-neo, PC3-PDGF, LNCaP-neo, and LNCaP-PDGF D cell lines. (A) Cells were grown to 100% confluence, washed with PBS, and cultured in serum-free medium for 48 h. CM was collected and resolved on an SDS-PAGE gel under denaturing conditions and then transferred to a nitrocellulose membrane. An antibody recognizing the beta chain of uPA was used for immunoblot analysis to detect the latent sc-uPA and the beta chain present in active, two-chain uPA. (B) Lysate was collected along with the CM used above, and 50 μg was resolved on an SDS-PAGE gel under reducing conditions, transferred to nitrocellulose, and probed with the same antibody used above.