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. 2005 Jul;25(14):5965–5972. doi: 10.1128/MCB.25.14.5965-5972.2005

FIG. 2.

FIG. 2.

GAC63 binds GRIP1 in vitro and in vivo. (A) In GST pull-down assays, GST fusion proteins of GRIP1N (amino acids 5 to 479) were bound to beads and incubated with in vitro-translated GAC63. Bound proteins were eluted and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autofluorography. (B) COS-7 cells were transfected with 2 μg of pcDNA3.1-GAC63-V5.His plasmid and 3 μg of pSG5.HA-GRIP1 plasmid. Cell extracts were immunoprecipitated with anti-GAC63 antibody or normal rabbit IgG. The immunoprecipitated proteins were detected by immunoblotting with anti-HA antibody. (C) COS-7 cells were transfected with 2 μg of pcDNA3.1-GAC63-V5.His plasmid and 2 μg of pSG5.HA-GRIP1(5-479) or pSG5.HA-GRIP1(563-1462). Cell extracts were immunoprecipitated with anti-His-tag antibody. The precipitated proteins were detected by immunoblotting with anti-HA antibody.