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. 2005 Jul;25(14):5965–5972. doi: 10.1128/MCB.25.14.5965-5972.2005

FIG. 4.

FIG. 4.

GRIP1-dependent NR coactivator function of GAC63. (A) CV-1 cells were transfected with 200 ng of mouse mammary tumor virus MMTV(ERE)-LUC reporter plasmid; 10 ng of pHE0; 50 ng of pSG5.HA-GRIP1; and 50, 100, or 200 ng of pSG5.HA-GAC63 and grown with or without E2. After 48 h, cell extracts were tested for luciferase activity. RLU, relative light units. (B) CV-1 cells were transfected with 200 ng of MMTV-LUC reporter plasmid, 10 ng of pSV-AR, 50 ng of pSG5.HA-GRIP1, and 200 ng of pSG5.HA-GAC63 and grown with or without DHT before luciferase assays. (C) CV-1 cells were transfected with 200 ng of MMTV(ERE)-LUC reporter plasmid, 10 ng of pHE0, 100 ng of pSG5.HA-GRIP1, or 50 ng of pSG5.GRIP1(563-1462) and 200 ng of pSG5.HA-GAC63 and grown with E2. After 48 h, cell extracts were tested for luciferase activity.