Synergy among GRIP1, CARM1, and GAC63. CV-1 cells were transfected with 200 ng of MMTV(ERE)-LUC reporter plasmid, 0.2 ng of pHE0, 50 ng of pSG5.HA-GRIP1, 200 ng of pSG5.HA-CARM1, 200 ng of pCMV-p300, and 200 ng of pSG5.HA-GAC63 and treated with E2 before harvest and luciferase assays. RLU, relative light units.