FIG. 7.

Recruitment of endogenous GAC63 to an endogenous E2-regulated promoter. (A) MCF-7 cells were grown with or without E2 for 60 min and analyzed by ChIP assays, using the indicated antibodies for immunoprecipitation and primers flanking the estrogen response element in the pS2 promoter for PCR analysis of the immunoprecipitated DNA. The results shown are representative of five independent experiments. (B) Real-time QPCR analysis of immunoprecipitated MCF-7 chromosomal DNA using primers for the pS2 promoter was performed with 2 μl of 1:10-diluted samples. The results are shown as percentage of input, are the mean and standard deviation from triplicate reactions, and are representative of two independent experiments.