FIG. 3.

CP2b/CP2c interaction increased α-globin gene expression in erythroid cells. K562 cells were transiently cotransfected with the pGL3-mouse α-globin promoter construct along with pcDNA3 (control), pcDNA3-CP2b, pcDNA3-CP2c, pEF1α-LSF-dominant negative for CP2c, or the pcDNA3-flag-CP2bΔ1-42 vector for the CP2b N-terminal deletion form in combinations as indicated. The total amount of DNA was adjusted to 600 ng for each transfection experiment. Luciferase activities shown are averages and standard deviation of three independent experiments.