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Age-dependent block in mast cell development in Mc-cpa−/− mice. Peritoneal cells were isolated from wild-type (Mc-cpa+/+ [+/+]) (A to C) and Mc-cpa−/− (−/−) (D to F) mice at the indicated age. Cells were cytospun and stained by alcian blue and safranin. Relative proportions of AB+++ S− (blue) (G), AB+++ S+ (blue > brown) (H), AB+ S+++ (brown > blue) (I), and AB− S+++ (brown) (J) staining (+++, strong staining; +, staining; −, no staining) are shown as a function of time.
