TABLE 2.
Hexosaminidase and histamine release from wild-type and mutant mast cellsa
| Genotypeb | % β-Hexosaminidase release (n)c
|
Histamine
|
|||
|---|---|---|---|---|---|
| Total amt (nmol/cell) (n)d | % Release (n)c
|
||||
| w/o | Ionomycin | w/o | Ionomycin | ||
| +/+ | 4.6 ± 2.4 (3) | 80.0 ± 5.1 (3) | 123.7 ± 3.1 (3) | 3.3 | 92.6 |
| −/− | 5.7 ± 1.0 (3) | 82.1 ± 10.6 (3) | 115.0 ± 22.6 (3) | 4.3 | 95.2 |
a
Cell sorter-purified mast cells from Mc-cpa+/+ and Mc-cpa−/− mice were left untreated or stimulated with ionomycin. Hexosaminidase and histamine were measured by colorimetric assay and by enzyme-linked immunosorbent assay, respectively.
b
+/+, Mc-cpa+/+; −/−, Mc-cpa−/−.
c
Release into the supernatant with or without ionomycin stimulation (w/o). Percentages of the release of hexosaminidase and histamine were calculated relative to the total hexosaminidase and histamine contents. n, number of mice.
d
The total amount of histamine was determined by comparison to a histamine standard curve. n, number of mice.