Table 2.
Comparison between the performance of microarray lateral flow device (µLFIA) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) in terms of recovery, repeatability, and reproducibility for aflatoxin B1 (AFB1), T-2 toxin (T2), zearalenone (ZEA), deoxynivalenol (DON), and fumonisin B1 (FB1) at final concentration of 10 (L1), 20 (L2), and 50 µg/kg (L3)
| Mycotoxins | Spiked level | µLFIA | LC–MS/MS | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Intra-assay | Inter-assay | Intra-assay | Inter-assay | ||||||
| Recovery (%) | RSD (%) | Recovery (%) | RSD (%) | Recovery (%) | RSD (%) | Recovery (%) | RSD (%) | ||
| AFB1 | L1 | 121 | 18 | 126 | 16 | 71 | 1 | 71 | 3 |
| L2 | 117 | 5 | 127 | 23 | 72 | 1 | 70 | 3 | |
| L3 | 112 | 22 | 106 | 21 | 80 | 1 | 79 | 2 | |
| T2 | L1 | 104 | 20 | 111 | 17 | 86 | 2 | 87 | 4 |
| L2 | 99 | 12 | 94 | 12 | 88 | 1 | 85 | 4 | |
| L3 | 116 | 12 | 94 | 18 | 102 | 1 | 97 | 5 | |
| ZEA | L1 | 127 | 22 | 121 | 23 | 81 | 1 | 80 | 4 |
| L2 | 105 | 14 | 105 | 13 | 79 | 1 | 78 | 1 | |
| L3 | 91 | 14 | 100 | 17 | 92 | 0 | 91 | 2 | |
| DON | L1 | 86 | 25 | 90 | 16 | 78 | 3 | 77 | 3 |
| L2 | 77 | 15 | 91 | 11 | 79 | 5 | 77 | 3 | |
| L3 | 108 | 22 | 88 | 19 | 85 | 1 | 84 | 2 | |
| FB1 | L1 | 93 | 13 | 97 | 15 | 72 | 3 | 70 | 9 |
| L2 | 99 | 10 | 104 | 11 | 69 | 2 | 70 | 2 | |
| L3 | 111 | 6 | 107 | 12 | 75 | 2 | 75 | 0 | |