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. 2024 Dec 31;7:1717. doi: 10.1038/s42003-024-07402-z

Fig. 5. Identification of the interacting partners of Frizzled-7 by Yeast two hybridization.

Fig. 5

a Screening of the yeast two-hybrid library using pBT3SUC-SjFrizz7 as the bait plasmid. NMY32 yeast cells containing the pBT3SUC-SjFizzled-7 bait plasmid transformed with plasmids from the Y2HSJ library and cultured on SDTLH + 5 mM 3AT plates. Colonies that grew on the selective plate were further inoculated onto SD-TL and SD-TLHA + 5 mM 3AT plates. b List of the interacting interactors identified by Y2H. c Re-transformation of SjFrizzled-7 bait plasmid and identified prey plasmids into yeast cells for confirming protein interactions. All diploid yeast strains for which the density was normalized before plating grew equally well under selection for both bait and prey plasmids (SD-Leu-Trp). d Diagram showing the protein-protein interaction network of Frizzled-7.