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. 2024 Dec 31;7:1717. doi: 10.1038/s42003-024-07402-z

Fig. 7. Yeast two-hybrid assay to screen the interacting partners of SjRho.

Fig. 7

a Re-transformation of SjRho- and its prey plasmids into yeast cells to confirm protein interactions. All diploid yeast strains for which the density was normalized before plating grew equally well under selection for both bait and prey plasmids (SD-Leu-Trp). Tenfold dilutions for each diploid sample were plated onto each selection plate. Colonies that grew on the selective plate were further inoculated onto SD-TLHA + 5 mM 3AT plates. pNubG-Fe65 + pTSU2-APP was used as a positive control, and pGADT7-BD+pGADT7 was used as a negative control. b List of identified interactors of SjRho by Y2H technique. c Effect of SjRho silencing on the expression of its interacting partners evaluated by RT-qPCR. Data are shown as the mean ± standard error, n = 3 independent experiments. * P ≤ 0.05, ns means non-significant.