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Kinetic parameters of wild type 3CLpro measured at different temperatures.
| ΔHapp (kcal mol-1) | KM (µM) | kcat (s-1) | kcat/KM (M-1 s-1) | |
|---|---|---|---|---|
| 298 K | -2.1 ± 0.1 | 81 ± 2 | 3.9 ± 0.1 | (48 ± 2) × 103 |
| 301 K | -2.2 ± 0.1 | 92 ± 1 | 4.8 ± 0.1 | (52 ± 2) × 103 |
| 304 K | -2.2 ± 0.1 | 100 ± 1 | 5.7 ± 0.1 | (57 ± 2) × 103 |
| 307 K | -2.0 ± 0.1 | 109 ± 1 | 7.6 ± 0.1 | (70 ± 2) × 103 |
| 310 K | -2.0 ± 0.1 | 124 ± 3 | 9.3 ± 0.1 | (75 ± 3) × 103 |
