Table 1.

Comparison of repeatability estimates of the PCR measurements using different reaction mixes and platforms over three days (using standard deviations of the Ct value).

Repeatability Estimates (standard deviations of the Ct value) based on Individual Plasmid Copy Number
Plasmid Copy Number	Platform and PCR Reaction Mix
	ABI 7700		LightCycler		RotorGene
	Excite	FastStart	Excite	FastStart	Excite	FastStart
102	0.543	0.182	0.568	0.233	0.888	0.186
103	0.452	0.204	1.177	0.190	0.184	0.445
104	0.223	0.305	1.123	0.100	0.256	0.592
105	0.243	0.168	0.947	0.224	0.209	0.221
106	0.362	0.183	1.378	0.281	0.772	0.333
107	0.473	0.189	0.919	0.156	0.319	0.247
108	0.236	0.184	0.454	0.352	0.322	0.166
Degrees of Freedom	6–8	6	6–8	5–6	6–8	6
Mean	0.362	0.202	0.938	0.219	0.421	0.313
Standard Deviation	0.130	0.047	0.331	0.082	0.286	0.156

(Note that one replicate is missing for the LC platform coupled with the Fast Start reaction mix – the capillary broke).