Figure 1.

Hel308 from archaea (mth810) interacts genetically with stalled replication forks. (A) Serial dilution of overnight cultures grown at 30°C showing growth of E.coli dnaE486 (lane a) that is severely restricted at 37°C (lane b) is improved by ΔrecQ (lane e). (B) Spot tests from overnight cultures grown at 30°C, showing that plasmid encoded archaeal gene mth810 stunts growth of dnaE486 ΔrecQ at 37°C, the same phenotype as plasmid encoded E.coli recQ. V is empty vector control and K51L denotes the mutation introduced into helicase motif I of mth810 (C) Mth810 has no dominant effect when transformed into wild-type or ΔrecQ strains; lanes a and d are empty vector; lanes b and e are recQ⁺; and lanes c and f are mth810.