Fig. 1.

AR-fluorescent protein fusions have normal responses to ligand. (A) Diagram of AR fusions to CFP and YFP. (B) An anti-AR antibody was used to probe nuclear (nuc) and cytoplasmic (cyto) extracts of HeLa cells expressing AR or CFP–AR–YFP (C-AR-Y) after treatment with DHT for different amounts of time. Quantification of band intensities (see Fig. 6B for blot) shows similar rates of ligand-induced nuclear transport for both. (C) MMTV-luciferase reporter activity increased when HEK293 cells expressing AR or AR fluorescent protein fusions were treated for 24 h with 100 nM DHT. The fold activity upon DHT addition is shown for each AR. Error bars represent the SEM.