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LacI bound to DNA in a nanochannel. (a) Time-averaged image of GFP–LacI bound to lacO256–DNA elongated in a 150 × 200 nm channel. There are ≈20 GFP–LacI molecules bound to this lacO256–DNA molecule. This molecule traveled from right to left into the nanochannel driven by an electric field of 5V/50 μm. (b) Fluorescence intensity profiles for the DNA and the bound GFP–LacI. The fits are dashed lines.
