FIG. 4.

Immunolocalization of UvsC::FLAG in germlings during S-phase recovery of the wild-type (ASG17) (A), scaA1 (GHG4) (B), and uvsB110 (ACS2) (C) strains. Mycelia of the ASG17, GHG4, and ACS2 strains were grown on coverslips in YG medium for 12 h. The coverslips were treated with 20 mM HU for 6 h, washed three times with prewarmed YG medium, and grown for 0, 30, 60, or 120 min after HU release. The adherent germlings were fixed, treated with an anti-FLAG antibody, and stained with Hoechst. The samples were analyzed by immunofluorescence microscopy, and distinct UvsC-FLAG subnuclear foci were counted (D). Images were obtained by laser scanning confocal microscopy. Left panels, nuclei stained by Hoechst; middle panels, UvsC::FLAG subnuclear foci; right panels, merged images.