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Stimulation of hyphal growth by cAMP. An aliquot of 2.5 × 107 cells was plated on LNB medium next to a 2.5.-cm filter disk soaked in 8-Br-cAMP (A and B, top frames) or with no disk (A and B, bottom frames). The cultures were incubated for 7 days, photographed (A), and washed and photographed again (B). Invasion is clearly visible after the addition of 8-Br-cAMP. (C and D) Higher-power images of the same hyphae. Bars = 400 μm (C) and 20 μm (D).
