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. 2005 Jul;4(7):1211–1220. doi: 10.1128/EC.4.7.1211-1220.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 7. — Enzymatic activity of recombinant CpSFP-PPT using [1-¹⁴C]acetyl-CoA and MBP-CpACP2 as cosubstrates. (A) Effects of cations (1.0 mM each) on the CpSFP-PPT activities. Inset shows the purified CpSFP-PPT (without S tag) analyzed by SDS-PAGE. (B) Effects of Mg²⁺ concentrations on the activity of CpSFP-PPT. (C) Enzyme kinetics of CpSFP-PPT determined using various concentrations of [1-¹⁴C]acetyl-CoA and 5.0 μM MBP-CpACP2 as cosubstrates. Note: In all samples, bars represent standard-error-of-the-mean values derived from at least three replicated reactions. The data presented here were generated from at least two independent experiments.