TABLE 1.

Transformation of CW15 and T61-9 with different DNA substrates^a

^aDNA molecules 1, 2, 5, 7, 9, and 10 generate transformants after HR or NHR, whereas 3, 4, 6, and 8 generate transformants only after HR. In the DNA form cloning, letters in parentheses refer to constructs of Fig. 1. In the Pm^r transformant column, the numbers of homologous recombinants are displayed in parentheses. If labeled with an asterisk, they have been analyzed by DNA blotting. The amount of DNA used for one experiment with 1 to 30 parallel transformations is given in μg; 1 to 3 μg was used per transformation.