TABLE 3.
Microarray analysis identified 30 genes down-regulateda during the mating process of C. albicans
| ORFb | Genec | Pheromone response in S. cerevisiaed | Function(s) |
|---|---|---|---|
| SAPs and LIPse | |||
| orf6.9036 | SAP3 | NA | Secreted aspartyl proteinase 3 |
| orf6.4644 | SAP1 | NA | Secreted aspartyl proteinase 1 |
| orf6.5210 | LIP2 | NA | Secretory lipase |
| Cell surface proteins | |||
| orf6.8640 | FLO9 | Induced | Similar to hypharegulated protein HYR1 |
| orf6.1042 | WSC2 | Induced | Cell wall integrity, stress response protein |
| orf6.3288 | YWP1 | NA | Putative cell wall protein |
| Metabolism | |||
| orf6.3413 | APE2 | No | Aminopeptidase |
| orf6.4694 | SUL1 | No | High-affinity sulfate transporter |
| orf6.2190 | SAM2 | No | S-adenosylmethionine synthetase 2 |
| orf6.5806 | LKH1 | Induced | Probable leukotriene A hydrolase |
| orf6.8469 | ALG8 | Repressed | Glycosyl transferase |
| orf6.6671 | PUT4 | R/I | Proline permease |
| orf6.9051 | THI4 | Induced | Thiazole biosynthetic enzyme precursor |
| orf6.1992 | SLC1 | Repressed | Fatty acyltransferase |
| orf6.6385 | COX11 | Repressed | Cytochrome c oxidase assembly protein |
| orf6.4023 | PFK26 | No | 6-Phosphofructose kinase |
| orf6.4161 | LEU42 | No | 2-Isopropylmalalate synthase |
| orf6.5274 | PFK2 | Repressed | Phosphofructokinase beta-subunit |
| orf6.1494 | HEM13 | Repressed | Oproporphyrinogen III oxidase |
| Other functions | |||
| orf6.6056 | OP4 | NA | Opaque-phase-specific protein |
| orf6.8863 | CHS7 | No | Probable membrane protein |
| orf6.5830 | TRM9 | Repressed | tRNA methyltransferase |
| orf6.4726 | CTF8 | No | Putative kinetochore protein |
| orf6.6713 | NA | Probable heat shock protease | |
| orf6.6475 | HSP31 | Induced | Heat shock protein |
| orf6.6564 | PSP1 | No | High-copy suppressor of TS mutant of DNA Polf |
| orf6.6535 | PTC4 | No | Ser/Thr protein phosphatase PP2C |
| orf6.8075 | HLJ1 | No | Tail-anchored ERg membrane protein, unknown function |
| Unknown functions | |||
| orf6.3229 | NA | ||
| orf6.6060 | NA |
See “Microarray analysis of gene expression in mating mixtures” in Results and Discussion.
ORF, open reading frame.
Names of C. albicans genes were obtained from the C. albicans genome database (http://www.genolist.pasteur.fr/CandidaDB). In case the genes were not annotated, the names of their S. cerevisiae orthologs were listed. If an unambiguous S. cerevisiae ortholog was not identified from the S. cerevisiae genome database (http://www.yeastgenome.org), the name of the closest ortholog was listed. -, unnamed sequence.
Pheromone response data of S. cerevisiae were obtained from Roberts et al. (46). Induced, demonstrated induction; No, demonstrated no induction; NA, not available (not reported or tested); Repressed, demonstrated repression; R/I, repressed/induced.
SAPs, secreted aspartyl proteinases; LIPs, lipases.
TS, mutant of DNA Pol, temperature-sensitive mutant of DNA polymerase.
ER, endoplasmic reticulum.