TABLE 5.
Shmooing in mating cultures and α-pheromone-treated cells derived from log- versus stationary-phase culturesa
Condition | Cell origin | Proportion of shmoos at 3 h (%) | Fold increase in cell number at 3 h | Proportion of singlets (%) at:
|
% Change after 3 h | Proportion of budding cells (%) at:
|
% Change after 3 h | ||
---|---|---|---|---|---|---|---|---|---|
0 h | 3 h | 0 h | 3 h | ||||||
a/a-α/α mating mixture | Log phase | 4 | 2.0 | 58 | 50 | −8 | 42 | 45 | +3 |
Stat.b phase | 25 | 2.4 | 92 | 60 | −30 | 8 | 15 | +7 | |
α-Pheromone-treated a/a cells | Log phase | 35 | 2.3 | 58 | 44 | −14 | 42 | 24 | −20 |
Stat. phase | 73 | 1.7 | 92 | 19 | −73 | 8 | 12 | +4 |
Log- and stationary-phase cells were diluted into fresh medium containing 3 × 10−6 M chemically synthesized α-pheromone (Lockhart et al. [35]) at a final cell density of 2 × 106 per ml.
Stat., stationary.