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. 2005 Jul;4(7):1273–1286. doi: 10.1128/EC.4.7.1273-1286.2005

FIG. 3.

FIG. 3.

GFP-Rsr1p and GFP-Bud2p localize to the cell cortex in yeast and hyphal cells. (A) C. albicans strains MG8393 (GFP-Rsr1p) and MG8063 (GFP-Bud2p) were grown under YF conditions and analyzed by GFP filter fluorescence and DIC microscopy. Scale bar, 2 μm. (B) C. albicans strain MG9232 (PGAL1-GFP-Rsr1p) was grown under YF (top; scale bar, 2 μm) or HF (bottom; scale bar, 5 μm) conditions and analyzed by GFP filter fluorescence and DIC microscopy. Localization of GFP-Rsr1p at a hyphal septum is indicated by an arrow. Adobe Photoshop v. 7 was used to process the acquired images in panels A and B. (C) Protein blots showing the relative levels of GFP-Rsr1p (top) and GFP-Bud2p (bottom) expressed in the strains shown in panels A and B. Strains MG8393 (lanes 1 and 2) and MG8063 (lanes 6 and 7) were grown in SDC medium at 28°C (YF) or in SDC medium plus 10% serum at 37°C (HF). Strain MG9232 (lanes 3 to 5) was grown in SDC medium lacking dextrose but containing 2% galactose at 28°C (YF), SC medium plus 2% galactose plus 10% serum at 37°C (HF), or in SDC medium at 28°C (off), as indicated.