FIG. 4.

Rsr1p and Bud2p are important for the polarization of actin patches in C. albicans YF cells. (A) C. albicans strains JB6284 (WT), CA8880 (rsr1Δ/Δ), and DH7453 (bud2Δ/Δ) were grown under YF conditions for 4 h. (B) S. cerevisiae strains JB1492 (WT), JB2762 (rsr1Δ), and JB2761 (bud2Δ) were incubated under YF conditions for 4 h. All strains were fixed with formaldehyde, stained with Alexa phalloidin to visualize actin, and photographed. The left panel in each pair is a DIC image; the right panel of each pair is a Texas red filter fluorescent image. Scale bar, 3 μm (all panels). Adobe Photoshop v. 7 was used to invert the acquired fluorescent images in order to enhance visualization.