TABLE 1.

Effect of protease inhibitors and additives on activity of P. marquandii and D. microsporus keratinases

Compound	Concn	Residual activity (%)
		Keratinase of P. marquandii	Keratinase of D. microsporus
Control		100	100
PMSF	1 mM	0	0
EDTA	5 mM	70	75
Iodoacetamide	0.05 mM	98	99
DTT	0.5 mM	143	175
	1 mM	230	322
	5 mM	197	266
β-ME	5 mM	108	160
	25 mM	115	215
SDS	0.1%	29	23
	0.5%	14	16
DMSO	1%	103	100
	5%	105	104
	10%	88	90
Isopropanol	1%	88	85