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Kinetic parameters for hydrolysis of AAPF with P. marquandii and D. microsporus keratinases and with proteinase K
| Enzyme | Km (mM) | Vmax (107 mmol/s) | kcat (s−1) | kcat/Km (mM−1s−1) |
|---|---|---|---|---|
| Keratinase of P. marquandii | 0.17 ± 0.02 | 7.3 ± 0.1 | 41.0 | 241 |
| Keratinase of D. microsporus | 1.03 ± 0.17 | 11.7 ± 0.4 | 8.8 | 9 |
| Proteinase K | 1.49 ± 0.30 | 8.2 ± 0.4 | 142.0 | 95 |
