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Disruption of the pksCT gene in M. purpureus. The strategy for disrupting the pksCT gene by a single crossover. A pCNS44 derivative containing the internal 2.1-kb SphI-KpnI (1) fragment (pCT1) was transformed into M. purpureus IFO30873. The single-crossover event between the Monascus genome and the SphI-KpnI (1) fragment on pCT1 results in truncated open reading frames that lack either the ACP domain or the KS domain.
