TABLE 4.
Phenotypic and genotypic characterization of 48 Y. enterocolitica strains isolated from yersiniosis patients
| Patient no. | Phenotypic characterization
|
Genotypic characterization
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Biotyping testsd
|
Biosero- groupa | CR-BHOb | PFGE (NotI)c | Multiplex PCR (yst rfbC ail virF)d | |||||
| Sal/Escul/Pyz | Xyl | Treh | VP | Lip | |||||
| 1, 16, 18, 22, 25, 42, 49, 52, 53, 54, 55, 58 | − | − | + | + | − | 4/O:3 | +++ | NA | + + + + |
| 39, 44, 46 | − | − | + | − | − | 4/O:3 | +++ | NA | + + + + |
| 32 | − | − | + | + | − | 4/O:3 | ++ | NA | + + + + |
| 13, 24, 40, 57 | − | − | + | + | − | 4/O:3 | + | NA | + + + + |
| 23, 28, 37, 48 | − | − | + | + | − | 4/O:3 | +++ | NB | + + + + |
| 4, 47 | − | − | + | + | − | 4/O:3 | + | NB | + + + + |
| 3 | − | − | + | + | − | 4/O:3 | ++ | NB | + + + + |
| 26 | − | − | + | − | − | 4/O:3 | +++ | NB | + + + + |
| 8, 56 | − | − | + | + | − | 4/O:3 | +++ | NB | + + + + |
| 7 | − | − | + | + | − | 4/O:3 | + | NE | + + + + |
| 11 | − | − | + | + | − | 4/O:3 | − | NE | + + + − |
| 14 | − | − | + | + | − | 4/O:3 | +++ | NI | + + + + |
| 5 | − | − | + | + | − | 4/O:3 | − | NI | + + + + |
| 21 | − | − | + | + | − | 4/O:3 | +++ | NG | + + + + |
| 29 | − | − | + | − | − | 4/O:3 | − | NH | + + + + |
| 17 | − | − | + | − | − | 4/O:3 | + | NC | + + + + |
| 27 | − | − | + | + | − | 4/O:3 | +++ | NF | + + + + |
| 9 | − | − | + | − | − | 4/O:3 | +++ | ND | + + + + |
| 2 | − | − | + | + | − | 4/O:3 | ++ | ND | + + + + |
| 2(200) | − | − | + | − | − | 4/O:3 | ++ | ND | + + + + |
| 19, 45 | − | − | + | + | − | 4/O:3 | + | ND | + + + + |
| 10 | − | − | + | + | − | 4/O:3 | ++ | NJ | + + + + |
| 34, 36, 50, 51 | + | + | + | + | + | IA | − | Nx | − − − − |
a
Biotyped according to the reduced biotyping schema proposed by the Wauters schema and serotyped using monovalent antiserum agglutination tests.
b
CR-BHO results: +/−, presence/absence of pinpoint colonies (numbers of visible pinpoint colonies are indicated as +, ≤10; ++, 10 to 150; or +++, >150).
c
Nx, NotI profiles different from those found in the Y. enterocolitica 4/O:3 strains.
d
+/−, positive and negative reactions, respectively. Sal, salicin; Escul, esculin; Pyz, pyrazinamidase; Xyl, xylose; Treh, trehalose; VP, Voges-Proskauer; Lip, lipase.