TABLE 5.

Phenotypic and genotypic characterization of seven pathogenic and four nonpathogenic Y. enterocolitica strains isolated from food and/or feed

Patient no.a	Strain no./food type	Phenotypic characterization									Genotypic characterization
		Biotyping testse							Bioserogroupb	CR-BHOc	PFGE (NotI)d	Multiplex PCR (yst rfbC ail virF)e
		Sal	Escul	Pyz	Xyl	Treh	VP	Lip
7	50A/minced pork	−	−	−	−	+	+	−	4/O:3	+++	NA	+ + + +
10	75A/minced pork	−	−	−	−	+	+	−	4/O:3	+++	NA	+ + + +
19	3a/ham	−	−	(+)	−	+	+	−	4/O:3	++	NJ	+ + + +
	5a/loin of pork	−	−	−	−	+	+	−	4/O:3	+++	ND	+ + + +
22	47/minced pork	−	−	−	−	+	+	−	4/O:3	+++	NA	+ + + +
32	237A1/fillet of pork	−	−	−	−	+	+	−	4/O:3	+++	NC	+ + + +
49	303/dog feed	−	−	−	−	+	+	−	4/O:3	+++	NI	+ + + +
11	43 1/pork chop	+	+	+	+	+	+	+	1A	−	Nx	− − − −
32	235A4/loin of pork	+	+	+	+	+	+	+	1A	−	Nx	− − − −
54	365A1/minced pork	+	+	+	+	+	+	+	1A	−	Nx	− − − −
54	366 2/ham	+	+	+	+	+	+	+	1A	−	Nx	− − − −

^aRefers to the patient numbers listed in Table 4.

^bBiotyped according to the reduced biotyping scheme proposed by Wauters et al. (34) and serotyped by using monovalent antisera agglutination tests.

^cCR-BHO results: +/−, presence/absence of pinpoint colonies (numbers of visible pinpoint colonies are indicated as +, ≤10; ++, 10 to 150; or +++, >150).

^dN^x, NotI profiles different from those found in the Y. enterocolitica 4/O:3 strains.

^e+/−, positive and negative reactions, respectively; (+), weak positive reaction. See footnote d of Table 4 for abbreviations.