TABLE 1.
Oligonucleotide primers and probe used
| Primer or probe | Sequence | Target (positions)a |
|---|---|---|
| BEV-F | 5′-GGGGAGTAGTCCGACTCCGC-3′ | 126-145 |
| BEV-R | 5′-CAGAGCTACCACTGGGGTTGTGG-3′ | 375-397 |
| NBEV-F | 5′-ACGGAGTAGATGGTATTCCC-3′ | 189-208 |
| NBEV-R | 5′-CGAGCCCCATCTTCCAGAG-3′ | 393-411 |
| BEV-5FL | 5′-GCCGTGAATGCTGCTAATCC-3′ | 533-552 |
| BEV-3FL | 5′-GTAGTCTGTTCCGCCTCCACCT-3′ | 604-625 |
| BEV-SON | 5′FAM-CGCACAATCCAGTGTTGCTACGTCGTAAC-3′TAMRAb | 570-598 |
a
The nucleotide numbering in the target sequence corresponds to that of the PS87 isolate (18).
b
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxy tetramethylrhodamine.