Nondenaturing PAGE of the purified EngL and the purified mini-CbpA1&2 complex. The complex EngL-mini-CbpA1&2 (molar ratio, 2:1) was incubated at 37°C. Lanes 3, 4, and 5 represent aliquots collected at 0, 10, and 20 h, respectively, and loaded in the 6% nondenaturing gel. Lane 1, EngL; lane 2, mini-CbpA1&2.