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. 2005 Jul;71(7):3504–3511. doi: 10.1128/AEM.71.7.3504-3511.2005

FIG. 4.

FIG. 4.

Purification of EngL, XynA, and mini-CbpA's and assembly of EngL and XynA cellulosomes. (A) SDS-PAGE analysis of purified XynA, EngL, and mini-CbpA's. Lane M, molecular marker; lane 1, EngL; lane 2, XynA; lane 3, mini-CbpA1&2; and lane 4, mini-CbpA5&6. (B) Interaction Western blot analysis. See Materials and Methods for details. Lane 1, mixture of EngL and mini-CbpA1&2; lane 2, mixture of EngL and mini-CbpA5&6; lane 3, mixture of XynA and mini-CbpA1&2; lane 4, mixture of XynA and mini-CbpA5&6.