TABLE 2.
Sequential reactions against CAX by ArfA and XynAa
| Added enzyme(s)
|
Liberated reducing sugar (μM)b | Synergyc | |
|---|---|---|---|
| In first reaction (time of reaction) | In second reaction (time of reaction) | ||
| ArfA (15 h) | XynA (5 h) | 52 (1.5) | 2.3 |
| XynA (15 h) | ArfA (5 h) | 55 (2.7) | 2.8 |
| Control reactions | |||
| XynA + ArfA (20 h) | 68 (1.0) | 2.4 | |
| ArfA (5 h) | 4 (1.0) | ||
| ArfA (20 h) | 12 (0.7) | ||
| XynA (5 h) | 11 (0.9) | ||
| XynA (20 h) | 16 (0.5) | ||
a
CAX (0.5%) was degraded by 0.5 μM concentrations of each enzyme at 37°C. The second enzyme was added immediately after the end of the incubation period of the first reaction.
b
The values are the averages of three determinations. The number in parentheses indicates standard deviation.
c
Synergy is as described in Table 1.