TABLE 3.
Sequential reactions against CAX by ArfA, XynA, EngE, and EngLa
| Added enzyme(s)
|
Liberated reducing sugar (μM)b | Synergyc | |
|---|---|---|---|
| In first reaction (time of reaction) | In second reaction (time of reaction) | ||
| ArfA + XynA (15 h) | EngE (5 h) | 168 (3.1) | 1.9 |
| EngE (15 h) | ArfA + XynA (5 h) | 114 (5.9) | 1.1 |
| ArfA + XynA (15 h) | EngL (5 h) | 105 (3.0) | 1.7 |
| EngL (15 h) | ArfA + XynA (5 h) | 112 (5.8) | 1.1 |
| Control reactions | |||
| XynA + ArfA + EngE (20 h) | 143 (1.9) | 1.3 | |
| XynA + ArfA + EngL (20 h) | 135 (2.0) | 1.3 | |
| ArfA (5 h) | 14 (0.1) | ||
| ArfA (20 h) | 17 (0.5) | ||
a
CAX (0.5%) was degraded by 0.8 μM concentrations of EngL, ArfA, and XynA and a 0.05 μM concentration of EngE at 37°C. The second enzyme was added immediately after the end of the incubation period of the first reaction.
b
The values are the average of three determinations. The number in parentheses indicates standard deviation.
c
Synergy is as described in Table 1.