TABLE 4.
Influence of the type of cellulose on the activity of EngL in the free state or complexed onto mini-scaffolding protein
| Substrate | Free EngL (μM) | EngL-mini-CbpA1&2
|
EngL-mini-CbpA5&6
|
||
|---|---|---|---|---|---|
| Amount (μM)a | SFb | Amount (μM)a | SFb | ||
| CMC | 115 (5.0) | 114 (7.3) | 1.0 | 110 (2.1) | 1.0 |
| AS-cellulose | 168 (6.1) | 182 (4.9) | 1.1 | 207 (10.5) | 1.2 |
| Avicel | 76 (2.9) | 104 (6.1) | 1.4 | 141 (5.2) | 1.9 |
a
The amount of released reducing sugars from each substrate (0.5%) at 37°C is given. Each enzyme and mini-scaffolding protein were in equimolar amounts. CMC, AS cellulose, and Avicel were degraded by 6 nM for 20 min, 0.1 μM for 20 h, and 0.25 μM for 20 h, respectively. The number in parentheses indicates standard deviation.
b
SF, ratio of reducing sugar released by mini-cellulosome to reducing sugars released by the corresponding free enzyme.