TABLE 6.
Sequential reactions against CAX by XynA and EngL attached to mini-CbpAa
| Added enzyme(s)
|
Liberated reducing sugar (μM) | Synergy | |
|---|---|---|---|
| In first reaction (time of reaction) | In second reaction (time of reaction) | ||
| XynA-mini-CbpA1&2 (15 h) | EngL-mini-CbpA1&2 (5 h) | 108 (5.0) | 1.4 |
| EngL-mini-CbpA1&2 (15 h) | XynA-mini-CbpA1&2 (5 h) | 97 (2.1) | 1.1 |
| XynA-mini-CbpA5&6 (15 h) | EngL-mini-CbpA5&6 (5 h) | 163 (0.9) | 1.3 |
| EngL-mini-CbpA5&6 (15 h) | XynA-mini-CbpA5&6 (5 h) | 144 (1.1) | 1 |
| Control reactions | |||
| XynA-mini-CbpA1&2 (5 h) | 24 (2.0) | ||
| XynA-mini-CbpA1&2 (20 h) | 24 (1.0) | ||
| XynA-mini-CbpA5&6 (5 h) | 19 (0.3) | ||
| XynA-mini-CbpA5&6 (20 h) | 21 (0.4) | ||
| EngL-mini-CbpA1&2 (5 h) | 52 (1.7) | ||
| EngL-mini-CbpA1&2 (20 h) | 60 (0.9) | ||
| EngL-mini-CbpA5&6 (5 h) | 105 (2.0) | ||
| EngL-mini-CbpA5&6 (20 h) | 134 (3.7) | ||
a
CAX (0.5%) was degraded by 0.1 μM EngL and XynA at 37°C for 20 h. Mini-scaffolding proteins (mini-CbpA1&2 or mini-CbpA5&6) were at 0.1 μM. The second enzyme was added immediately after the end of the incubation period of the first reaction. The number in parentheses indicates standard deviation.