TABLE 1.
Plasmids
| Plasmid | Description | Source (reference) |
|---|---|---|
| pRY112 | Shuttle vector | P. Guerry (34) |
| pMW10-13 | Shuttle vector containing wild-type gfp gene | E. Gaynor (unpublished) |
| pMEK91 | Shuttle vector containing egfp-pMEK under control of ompE promoter | M. E. Konkel (24) |
| pAV35 | Source of Camr gene cassette | A. van Vliet (29) |
| pJMK30 | Source of Kanr gene cassette | A. van Vliet (29) |
| pGUO0202 | Shuttle vector | V. Korolik (1) |
| pGEM-T Easy | Cloning vector | Promega, Southampton, United Kingdom |
| pEGFP | Contains egfp gene | BD Biosciences Clontech, Palo Alto, CA |
| pRR | Fragment of rRNA gene cluster cloned into pGEM-T Easy | This study |
| pRRC | Camr cassette cloned into pRR | This study |
| pRPGL | pglH gene in pRRC | This study |
| pRMAF | maf5 gene in pRRC | This study |
| pRGW | Wild-type gfp gene in pRRC | This study |
| pRED | egfp gene from pEGFP with added optimal SD sequence cloned into pRRC | This study |
| pREM | PompE-egfp fusion from pMEK91 in pRRC | This study |