TABLE 2.
Estimated enterovirus concentrations in river samples as determined by cell culture, RT-PCR, and NASBA
| Sample | River | PFU/liter in cell culture | Method I (2PHS + Boom) RT-PCR at PDU/liter (p2.5-p97.5)b | Method II (UF + Magnetic extraction)
|
|
|---|---|---|---|---|---|
| RT-PCR at PDU/liter (p2.5-p97.5)b | NASBA at NDU/liter (p2.5-p97.5)b | ||||
| 02-8a | A | 1.4 | 2.6 (0.15-11.6) | 1,340 (308-5,165) | 9,548 (1,249-72,843) |
| 02-9a | A | 1.35 | 0.88 (0.21-2.5) | 91.5 (19.5-358) | 388 (76.0-1,304) |
| 03-2a | A | 0 | 0 (0-0.05) | 0 (0-20.0) | 0 (0-28.9) |
| 04-1a | B | 0.26 | 0 (0-0.08) | 1,317 (282-4,380) | 0 (0-82.9) |
| 04-2a | B | 0.17 | 0 (0-0.04) | 189 (43.6-726) | 32.9 (5.4-104) |
| 01-1 | C | 0.31 | 1,390 (244-5,753) | 826 (107-5,978) | |
| 01-2 | C | 0.27 | 168 (26.6-762) | 178 (20.2-824) | |
| 01-3 | C | 0.80 | 97.3 (13.7-502) | 435 (77.6-1,479) | |
| 01-4 | C | 0.90 | 144 (22.7-649) | 152 (17.2-702) | |
| 01-5 | C | 0.19 | 0 (0-31.5) | 0 (0-57.8) | |
| 01-6 | C | 0.65 | 55.0 (7.7-284) | 706 (91.6-5,110) | |
| 01-7 | C | 0.35 | 68.1 (11.0-224) | 346 (39.2-1,602) | |
| 03-3 | A | 0.12 | 30.5 (1.7-141) | 0 (0-48.9) | |
| 04-3 | D | 0.56 | 241 (33.8-1,243) | 0 (0-95.5) | |
| 04-4 | D | 0 | 0 (0-26.0) | 0 (0-26.2) | |
a
Samples described in Table 1.
b
95% confidence intervals are given in parentheses.