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. 2005 Jul;71(7):3709–3715. doi: 10.1128/AEM.71.7.3709-3715.2005

FIG. 5.

FIG. 5.

Effects of EDTA on AmyA under different buffer conditions. The enzyme was diluted to 50 μg ml−1 with the corresponding buffer and preincubated with CaCl2 or EDTA at 60°C for 1 h. Black bars, AmyA without additive; light-gray bars, AmyA with 5 mM EDTA; white bars, AmyA with 5 mM CaCl2; dark-gray bar, AmyA with 5 mM EDTA plus 10 mM CaCl2 added to the activity test. The effect of CaCl2 in MacIlvaine buffer was not determined due to precipitation.