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. 2005 Jul;71(7):4101–4103. doi: 10.1128/AEM.71.7.4101-4103.2005

FIG. 3.

FIG. 3.

SDS-PAGE and zymogram analysis of MPH production by M6 and WB800 carrying pUBC19 and pP43NMK. The supernatant of the M6 cell fraction and the culture supernatant of WB800 harboring different vectors were analyzed on a 12% SDS-polyacrylamide gel. The partial gel containing lanes 5 to 7 was excised for the zymogram analysis, and the remaining gel was stained with Coomassie brilliant blue R250. Lane 1, molecular mass markers; lanes 2 and 5, M6; lanes 3 and 6, WB800(pUBC19) (negative control); lanes 4 and 7, WB800(pP43NMK).