FIG. 1.

Detection of OASS-A, MalY, and OASS-B by CD activity staining. Preparation of native PAGE gel, cell extracts, and CD activity staining were carried out according to the method described previously (2). Lane 1, wild-type JM39 harboring pBluescript II SK+ (vector control; Toyobo Biochemicals, Osaka, Japan); lane 2, JM39 harboring pcysK (cysK plasmid; OASS-A is overexpressed); lane 3, JM39 harboring pcysM (cysM plasmid; OASS-B is overexpressed); lane 4, JM39 harboring pmalY (malY plasmid; MalY is overexpressed).